BCR-ABL


The BCR-ABL fusion results in constitutive ABL tyrosine kinase activity contributing to unregulated cell division. The BCR-ABL fusion is found in 95% of chronic myelogenous leukemia (CML), 25–30% adult acute lymphoblastic leukemia (ALL), and 2–10% child ALL. Researchers have identified the utility of measuring BCR-ABL transcripts to aid in the assessment of minimal residual disease (MRD) and the response to treatment. An international consortium has developed “International Standards” allowing the comparison of BCR-ABL levels measured by different quantitative PCR assays.

CytoGenes offers primer mixes, standards, and controls allowing laboratories to detect the three most common BCR-ABL fusions by quantitative PCR (b2a2, b3a2, and e1a2). A set of standards normalized to the International Scale allows results to be reported on the International Scale.

The graphs below demonstrate the high degree of correlation between the NIBSC and CytoGenes International Scale Standards for both the b2a2 and b3a2 BCR-ABL fusion products.

 

References

 

  

Additional Product Information

 
    • White HE, Matejtschuk P, Rigsby P, Gabert J, Lin F, Lynn Wang Y, Branford S, Müller MC, Beaufils N, Beillard E, Colomer D, Dvorakova D, Ehrencrona H, Goh HG, El Housni H, Jones D, Kairisto V, Kamel-Reid S, Kim DW, Langabeer S, Ma ES, Press RD, Romeo G, Wang L, Zoi K, Hughes T, Saglio G, Hochhaus A, Goldman JM, Metcalfe P, Cross NC. Establishment of the first World Health Organization International Genetic Reference Panel for quantitation of BCR-ABL mRNA. Blood. 2010 Nov 25;116(22):e111-7. Epub 2010 Aug 18.

 

    • WHO International Standard. 1st WHO International Genetic Reference Panel for quantitation of BCR-ABL translocation by RQ-PCR.

 

  • Hughes T, et al. Monitoring CML patients responding to treatment with tyrosine kinase inhibitors: Review and recommendations for harmonizing current methodology for detecting BCR-ABL transcripts and kinase domain mutations and for expressing results. Blood. 2006;108:28-37.

BCR-ABL Primer Mixes Product Data Sheet

BCR-ABL Controls Product Data Sheet

BCR-ABL Standards Product Data Sheet

BCR-ABL International Scale Standards

BCR-ABL Suggested Protocol